Cytotoxicity, MEM extracts, MTT test system, 24 hrs.
| Table 1 |
: |
Confluency of monolayer |
| Grade 0 |
: |
100% |
|
| Grade 1 |
: |
90-100% |
|
| Grade 2 |
: |
60-90% |
|
| Grade 3 |
: |
30-60% |
|
| Grade 4 |
: |
0-30% |
|
| Table 2 |
: |
Change of cell morphology |
| Grade 0 |
: |
no changes, normal cel |
| Grade 1 |
: |
slight changes, few cells affected |
| Grade 2 |
: |
mild changes, some cells rounded and/or spindle shaped |
| Grade 3 |
: |
moderate changes, many cells rounded and/or spindle shaped |
| Grade 4 |
: |
severe changes, about all cells show morphologic change. |
| Table 3 |
: |
Inhibition of cell metabolism |
| Grade 0 |
: |
0-10% |
compared to the negative control |
| Grade 1 |
: |
10-30% |
compared to the negative control |
| Grade 2 |
: |
30-50% |
compared to the negative control |
| Grade 3 |
: |
50-70% |
compared to the negative control |
| Grade 4 |
: |
70-100% |
compared to the negative control |
|
|
|
|
| Table 4 : Acceptance Criteria |
| cytotoxic |
response reactivity |
pass/fail |
|
|
| 0.0 - 1.0 |
none |
pass |
|
| 1.1 - 3.0 |
slight |
pass |
|
| 3.1 - 5.0 |
mild |
retest |
|
| 5.1 - 7.0 |
moderate |
fail |
|
| 7.1 - 8.0 |
severe |
fail |
|
|